1Q1R
Crystal Structure of Putidaredoxin Reductase from Pseudomonas putida
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL7-1 |
| Synchrotron site | SSRL |
| Beamline | BL7-1 |
| Temperature [K] | 200 |
| Detector technology | IMAGE PLATE |
| Collection date | 2001-04-01 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.08 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 70.780, 104.130, 166.780 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 50.000 * - 1.900* |
| Rwork | 0.213 |
| R-free | 0.23600 * |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1dy7 |
| RMSD bond length | 0.006 |
| RMSD bond angle | 24.300 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 29.570 | 1.930 |
| High resolution limit [Å] | 1.900 * | 1.910 |
| Rmerge | 0.047 | 0.568 |
| Number of reflections | 90180 * | |
| <I/σ(I)> | 16.8 | 1.5 |
| Completeness [%] | 99.2 | 97.7 |
| Redundancy | 3.5 | 1.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8 | 298 | lithium sulfate, lithium acetate, lithium formate, bis-tris propane, glycerol, dithiothreitol, pH 8.0, VAPOR DIFFUSION, SITTING DROP, temperature 298K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 25-30 (mg/ml) | |
| 2 | 1 | drop | sodium phosphate | 10 (mM) | pH8.0 |
| 3 | 1 | reservoir | lithium sulfate | 1.5 (M) | |
| 4 | 1 | reservoir | lithium acetate | 0.15 (M) | |
| 5 | 1 | reservoir | lithium formate | 0.1 (M) | |
| 6 | 1 | reservoir | glycerol | 2 (%(v/v)) | |
| 7 | 1 | reservoir | dithiothreitol | 1 (mM) | |
| 8 | 1 | reservoir | Tris-propane | 0.1 (M) | pH8.0 |
