1Q1K
Structure of ATP-phosphoribosyltransferase from E. coli complexed with PR-ATP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SRS BEAMLINE PX9.6 |
| Synchrotron site | SRS |
| Beamline | PX9.6 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2002-07-05 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.86 |
| Spacegroup name | H 3 2 |
| Unit cell lengths | 132.100, 132.100, 113.379 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 50.000 - 2.900 |
| R-factor | 0.21454 |
| Rwork | 0.210 |
| R-free | 0.27243 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1h3d |
| RMSD bond length | 0.018 |
| RMSD bond angle | 2.018 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.1.24) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.020 |
| High resolution limit [Å] | 2.900 | 2.900 |
| Rmerge | 0.058 | 0.581 |
| Number of reflections | 8521 | |
| <I/σ(I)> | 16.9 | 2 |
| Completeness [%] | 99.3 | 99.9 |
| Redundancy | 4.2 | 4.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.6 | 298 | sodium tartrate, magnesium chloride, sodium citrate, pH 5.6, VAPOR DIFFUSION, SITTING DROP, temperature 298K |
