1Q1H
An extended winged helix domain in general transcription factor E/IIE alpha
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06SA |
Synchrotron site | SLS |
Beamline | X06SA |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2003-05-02 |
Detector | MARRESEARCH |
Wavelength(s) | 0.9793 |
Spacegroup name | P 61 2 2 |
Unit cell lengths | 66.100, 66.100, 92.700 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 6.500 - 2.900 |
R-factor | 0.3151 |
Rwork | 0.315 |
R-free | 0.32100 |
Structure solution method | MAD |
RMSD bond length | 0.007 |
RMSD bond angle | 1.330 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | SOLVE |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | |
High resolution limit [Å] | 2.900 | 2.900 * |
Rmerge | 0.077 * | 0.285 * |
Number of reflections | 3025 | 292 * |
Completeness [%] | 98.7 | 100 * |
Redundancy | 9.1 * |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 * | 293 | TRIS-Cl, sodium acetate, PEG 1000, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | HEPES | 50 (mM) | pH7.5 |
2 | 1 | drop | 200 (mM) | ||
3 | 1 | drop | EDTA | 1 (mM) | |
4 | 1 | drop | 10000 (nM) | ||
5 | 1 | drop | dithiothreitol | 5 (mM) | |
6 | 1 | drop | protein | 15 (mg/ml) | |
7 | 1 | reservoir | Tris-Cl | 100 (mM) | pH8.5 |
8 | 1 | reservoir | 200 (mM) | ||
9 | 1 | reservoir | PEG4000 | 30 (%(w/v)) |