1Q1F
Crystal structure of murine neuroglobin
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ELETTRA BEAMLINE 5.2R |
Synchrotron site | ELETTRA |
Beamline | 5.2R |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2003-06-12 |
Detector | MARRESEARCH |
Wavelength(s) | 1.2, 1.7 |
Spacegroup name | H 3 2 |
Unit cell lengths | 86.973, 86.973, 110.815 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 20.000 - 1.500 |
R-factor | 0.21 |
Rwork | 0.184 |
R-free | 0.20750 |
Structure solution method | SAD |
Data reduction software | DENZO |
Data scaling software | CCP4 ((TRUNCATE)) |
Phasing software | SOLVE |
Refinement software | REFMAC |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 40.000 | 1.550 |
High resolution limit [Å] | 1.500 | 1.500 |
Rmerge | 0.074 | 0.536 |
Number of reflections | 26076 | |
<I/σ(I)> | 35 | 2.79 |
Completeness [%] | 99.5 | 100 |
Redundancy | 10.8 | 7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 4.6 | 293 | 0.1 M Na acetate, 2M Na formate, pH 4.6, VAPOR DIFFUSION, HANGING DROP, temperature 293K |