1Q1B
Crystal structure of E. coli MalK in the nucleotide-free form
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X4A |
Synchrotron site | NSLS |
Beamline | X4A |
Temperature [K] | 200 |
Detector technology | CCD |
Collection date | 2001-11-11 |
Wavelength(s) | 0.97 |
Spacegroup name | P 1 |
Unit cell lengths | 60.617, 93.540, 99.796 |
Unit cell angles | 77.63, 79.38, 73.20 |
Refinement procedure
Resolution | 20.000 * - 2.800 |
R-factor | 0.249 |
Rwork | 0.249 |
R-free | 0.30400 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.010 |
RMSD bond angle | 24.700 * |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | AMoRE |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 * | 2.900 |
High resolution limit [Å] | 2.800 | 2.800 |
Rmerge | 0.058 * | 0.265 * |
Number of reflections | 51331 * | 5142 * |
Completeness [%] | 97.8 * | 98.4 * |
Redundancy | 1.8 | 1.9 * |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8 * | 293 | PEG 4000, glycerol, Sodium Acetate, Tris, pH 8.8, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 10 (mg/ml) | |
2 | 1 | drop | ATP | 1 (mM) | |
3 | 1 | drop | EDTA | 1 (mM) | |
4 | 1 | drop | dithiothreitol | 10 (mM) | |
5 | 1 | drop | glycerol | 20 (%) | |
6 | 1 | drop | sodium citrate | 10 (mM) | pH8. |
7 | 1 | reservoir | PEG5000 MME | 24 (%) | |
8 | 1 | reservoir | ammonium sulfate | 0.2 (M) | |
9 | 1 | reservoir | MES | 100 (mM) | pH6.5 |