1Q1A
Structure of the yeast Hst2 protein deacetylase in ternary complex with 2'-O-acetyl ADP ribose and histone peptide
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CHESS BEAMLINE F1 |
| Synchrotron site | CHESS |
| Beamline | F1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2003-02-20 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.9 |
| Spacegroup name | H 3 |
| Unit cell lengths | 98.421, 98.421, 77.254 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 29.740 - 1.500 |
| R-factor | 0.183 |
| Rwork | 0.183 |
| R-free | 0.20900 * |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1q14 |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.220 * |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.550 |
| High resolution limit [Å] | 1.500 | 1.500 |
| Rmerge | 0.049 | 0.172 |
| Number of reflections | 43331 * | |
| <I/σ(I)> | 20.9 | 4.2 |
| Completeness [%] | 96.9 | 93.9 |
| Redundancy | 6.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.6 | 294 | PEG 4K, , pH 5.6, VAPOR DIFFUSION, HANGING DROP, temperature 294K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 10 (mg/ml) | |
| 2 | 1 | reservoir | 0.2 (M) | ||
| 3 | 1 | reservoir | sodium citrate | 100 (mM) | pH5.6 |
| 4 | 1 | reservoir | PEG4000 | 25 (%) |
