1Q18

Crystal structure of E.coli glucokinase (Glk)

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X8C
Synchrotron site	NSLS
Beamline	X8C
Temperature [K]	100
Detector technology	CCD
Collection date	2003-06-15
Detector	ADSC QUANTUM 4
Wavelength(s)	0.964711, 0.979962, 0.980178
Spacegroup name	P 43 21 2
Unit cell lengths	81.474, 81.474, 234.710
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.760 - 2.360
R-factor	0.20931
Rwork	0.206
R-free	0.26700
Structure solution method	MAD
RMSD bond length	0.009
RMSD bond angle	1.226
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	SOLVE
Refinement software	REFMAC (5.1.24)

Data quality characteristics

	Overall
Low resolution limit [Å]	19.760
High resolution limit [Å]	2.360
Number of reflections	30408
Completeness [%]	95.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.5	294	20% PEG 4000, 0.1M Tris-HCl, 0.2M MgCl2, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 294K