1Q06
Crystal structure of the Ag(I) form of E. coli CueR, a copper efflux regulator
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 5ID-B |
Synchrotron site | APS |
Beamline | 5ID-B |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2002-04-23 |
Detector | MARRESEARCH |
Wavelength(s) | 1.000 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 59.516, 66.583, 81.821 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 24.070 - 2.070 |
R-factor | 0.219 |
Rwork | 0.219 |
R-free | 0.25900 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | Au-CueR |
RMSD bond length | 0.005 |
RMSD bond angle | 1.000 |
Data reduction software | MOSFLM |
Data scaling software | CCP4 ((SCALA)) |
Phasing software | CNS |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 24.070 | 2.130 |
High resolution limit [Å] | 2.070 | 2.070 |
Rmerge | 0.050 | 0.295 |
Number of reflections | 20248 | |
<I/σ(I)> | 10.8 | 3.1 |
Completeness [%] | 99.3 | 91.9 |
Redundancy | 5.3 | 3.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 291 | sodium citrate, sodium cacodylate, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 291K |