1PZN
Rad51 (RadA)
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL9-2 |
Synchrotron site | SSRL |
Beamline | BL9-2 |
Detector technology | CCD |
Collection date | 2002-04-07 |
Detector | ADSC |
Wavelength(s) | 0.979029 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 144.163, 193.124, 176.930 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 40.000 * - 2.850 |
Rwork | 0.257 |
R-free | 0.30700 |
Structure solution method | MAD |
RMSD bond length | 0.009 * |
RMSD bond angle | 1.390 * |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | SHARP |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 40.000 | 2.950 |
High resolution limit [Å] | 2.850 | 2.850 |
Rmerge | 0.094 * | 0.370 * |
Number of reflections | 462283 * | 57867 * |
<I/σ(I)> | 20.3 | 3.2198 |
Completeness [%] | 97.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion, sitting drop * | |||
1 | Vapor diffusion, sitting drop * |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 0.2 (mg/ml) | |
2 | 1 | drop | ATP | 1 (mM) | |
3 | 1 | drop | 20 (mM) |