1PZL
Crystal structure of HNF4a LBD in complex with the ligand and the coactivator SRC-1 peptide
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X12C |
Synchrotron site | NSLS |
Beamline | X12C |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2002-11-11 |
Detector | BRANDEIS - B2 |
Wavelength(s) | 0.97904 |
Spacegroup name | P 41 21 2 |
Unit cell lengths | 81.491, 81.491, 104.709 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 20.000 - 2.100 |
R-factor | 0.229 |
Rwork | 0.226 |
R-free | 0.26700 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.006 |
RMSD bond angle | 1.078 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | MOLREP |
Refinement software | CNS (1.0) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 29.900 | 2.140 |
High resolution limit [Å] | 2.100 | 2.100 |
Number of reflections | 20180 | |
<I/σ(I)> | 32.7 | 3.59 |
Completeness [%] | 95.1 | 76.7 |
Redundancy | 12 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 294 | Sodium, Potassium Tartrate, Hepes, DTT, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 294K |