1PZE
T.gondii LDH1 apo form
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL9-1 |
| Synchrotron site | SSRL |
| Beamline | BL9-1 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2002-01-04 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.97 |
| Spacegroup name | I 41 2 2 |
| Unit cell lengths | 77.459, 77.459, 218.577 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.000 - 1.950 |
| R-factor | 0.188 |
| Rwork | 0.188 |
| R-free | 0.22200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ldg |
| RMSD bond length | 0.016 * |
| RMSD bond angle | 1.640 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | EPMR |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.980 |
| High resolution limit [Å] | 1.950 | 1.950 |
| Rmerge | 0.043 | 0.349 |
| Total number of observations | 141594 * | |
| Number of reflections | 24776 | |
| <I/σ(I)> | 4.5 | |
| Completeness [%] | 99.9 | 99.8 |
| Redundancy | 5.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion * | 4.2 | 293 | 24% PEG 1K, 100 mM phosphate-citrate, pH 4.2, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 6 (mg/ml) | |
| 2 | 1 | drop | gossylic iminolactone | 1 (mM) | |
| 3 | 1 | drop | PEG1000 | 12 (%(w/v)) | |
| 4 | 1 | drop | phosphate-citrate | 50 (mM) | pH4.2 |
| 5 | 1 | reservoir | PEG1000 | 24 (%(w/v)) | |
| 6 | 1 | reservoir | phosphate-citrate | 100 (mM) | pH4.2 |
