1PZC

APO-PSEUDOAZURIN (METAL FREE PROTEIN)

Experimental procedure

Source type	SYNCHROTRON
Source details	EMBL/DESY, HAMBURG BEAMLINE BW7B
Synchrotron site	EMBL/DESY, HAMBURG
Beamline	BW7B
Detector technology	IMAGE PLATE
Collection date	1994-07-13
Detector	MARRESEARCH
Wavelength(s)	0.87
Spacegroup name	P 65
Unit cell lengths	49.500, 49.500, 99.000
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	10.000 - 1.850
R-factor	0.164
R-free	0.21500
RMSD bond length	0.016 *
RMSD bond angle	0.040 *
Data reduction software	DENZO
Refinement software	PROLSQ

Data quality characteristics

	Overall
Low resolution limit [Å]	10.000 *
High resolution limit [Å]	1.850 *
Rmerge	0.077
Total number of observations	73694 *
Number of reflections	11516 *
Completeness [%]	98.4
Redundancy	6.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion *	5.7 *		Petratos, K., (1987) FEBS Lett., 218, 209. *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	1.5 (%)
2	1	drop		50 (mM)
3	1	drop	ammonium sulfate	1.5 (M)
4	1	reservoir	ammonium sulfate	3.0 (M)