1PZ9
Modulation of agrin function by alternative splicing and Ca2+ binding
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE BW7B |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | BW7B |
| Wavelength(s) | 1.0 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 102.169, 56.751, 74.889 |
| Unit cell angles | 90.00, 127.04, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.800 |
| R-factor | 0.2399 |
| Rwork | 0.238 |
| R-free | 0.27105 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.605 |
| Data reduction software | MOSFLM |
| Data scaling software | CCP4 ((SCALA)) |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.0) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 30.900 |
| High resolution limit [Å] | 2.780 |
| Number of reflections | 8472 |
| <I/σ(I)> | 13.5 |
| Completeness [%] | 99.2 |
| Redundancy | 3.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 276 | PEG 8000, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 276K |
