1PZ4
The structural determination of an insect (mosquito) Sterol Carrier Protein-2 with a ligand bound C16 Fatty Acid at 1.35 A resolution
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU RU200 |
Temperature [K] | 293 |
Detector technology | AREA DETECTOR |
Detector | Bruker X1000 |
Wavelength(s) | 1.54178 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 29.300, 46.100, 70.400 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 30.000 * - 1.350 |
R-factor | 0.187 |
Rwork | 0.187 |
R-free | 0.22600 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.012 |
RMSD bond angle | 2.234 * |
Data reduction software | SAINT |
Data scaling software | SAINT |
Phasing software | EPMR |
Refinement software | TNT |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 * | 1.410 |
High resolution limit [Å] | 1.350 | 1.350 |
Rmerge | 0.038 | 0.252 |
Total number of observations | 78412 * | |
Number of reflections | 21157 | 2383 * |
<I/σ(I)> | 3.3 | |
Completeness [%] | 97.0 | 91.5 * |
Redundancy | 3.7 | 2.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | 2M sodium malonate, 100 mM Tris-HCl, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | 10 (mg/ml) | ||
2 | 1 | reservoir | 2 (M) | ||
3 | 1 | reservoir | 100 (mM) | pH7.5 |