1PYT

TERNARY COMPLEX OF PROCARBOXYPEPTIDASE A, PROPROTEINASE E, AND CHYMOTRYPSINOGEN C

Experimental procedure

Detector technology	IMAGE PLATE
Detector	MARRESEARCH
Spacegroup name	H 3
Unit cell lengths	188.500, 188.500, 82.500
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	6.000 - 2.350
R-factor	0.192
Rwork	0.192
R-free	0.28300
RMSD bond length	0.012
RMSD bond angle	1.822
Data reduction software	MOSFLM
Phasing software	X-PLOR
Refinement software	X-PLOR

Data quality characteristics

	Overall
Low resolution limit [Å]	20.000
High resolution limit [Å]	2.350
Rmerge	0.078
Number of reflections	39633
Completeness [%]	87.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, sitting drop *	7.1 *	4 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop		0.1 (M)
2	1	drop	HEPES	0.05 (M)
3	1	drop	PEG400	15 (%(v/v))
4	1	drop	protein	15 (mg/ml)
5	1	reservoir		0.1 (M)
6	1	reservoir	HEPES	0.05 (M)
7	1	reservoir	PEG400	15 (%(v/v))