1PYI
CRYSTAL STRUCTURE OF A PPR1-DNA COMPLEX: DNA RECOGNITION BY PROTEINS CONTAINING A ZN2CYS6 BINUCLEAR CLUSTER
Experimental procedure
| Detector technology | AREA DETECTOR |
| Collection date | 1993-10-09 |
| Detector | XENTRONICS |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 53.800, 92.600, 174.700 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 10.000 - 3.200 |
| R-factor | 0.245 |
| R-free | 0.33000 |
| RMSD bond length | 0.017 * |
| RMSD bond angle | 3.700 * |
| Data reduction software | XDS |
Data quality characteristics
| Overall | |
| Rmerge | 0.064 |
| Number of reflections | 7096 |
| Redundancy | 4.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 6.8 * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein dimer | 0.6 (mM) | |
| 10 | 1 | reservoir | MPD | 2 (%) | |
| 11 | 1 | reservoir | 125 (mM) | ||
| 12 | 1 | reservoir | zinc acetate | 0.100 (mM) | |
| 13 | 1 | reservoir | sodium cacodylate | 20 (mM) | pH6.8 |
| 2 | 1 | drop | DNA duplex | 0.7 (mM) | |
| 3 | 1 | drop | PEG3500 | 7 (%) | |
| 4 | 1 | drop | MPD | 1 (%) | |
| 5 | 1 | drop | 87.5 (mM) | ||
| 6 | 1 | drop | 62.5 (mM) | ||
| 7 | 1 | drop | zinc acetate | 0.050 (mM) | |
| 8 | 1 | drop | sodium cacodylate | 0.010 (mM) | pH6.8 |
| 9 | 1 | reservoir | PEG3500 | 15 (%) |
