1PYH
Crystal structure of RC-LH1 core complex from Rhodopseudomonas palustris
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SRS BEAMLINE PX14.1 |
| Synchrotron site | SRS |
| Beamline | PX14.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2002-02-06 |
| Detector | ADSC QUANTUM 4 |
| Spacegroup name | P 1 |
| Unit cell lengths | 76.037, 119.024, 130.432 |
| Unit cell angles | 69.32, 72.69, 66.52 |
Refinement procedure
| Resolution | 60.000 - 4.800 |
| R-factor | 0.4687 |
| Rwork | 0.467 |
| R-free | 0.48400 * |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | REACTION CENTRE |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 60.000 | 4.970 |
| High resolution limit [Å] | 4.800 | 4.800 |
| Rmerge | 0.086 | 0.721 |
| Total number of observations | 37214 * | |
| Number of reflections | 18335 * | |
| <I/σ(I)> | 8.3 | 1.1 |
| Completeness [%] | 97.3 | 83.4 |
| Redundancy | 2 | 2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, sitting drop * | 8.5 | 10-16 * | MMe PEG 2000, sucrose monocholate, magnesium chloride, spermidine, TRIS HCl, pH 8.5, VAPOR DIFFUSION, SITTING DROP, temperature 283-289K, pH 8.50 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | sucrose monocholate | 0.5 (%(w/v)) | |
| 2 | 1 | drop | spermidine | 1 (%(w/v)) | |
| 3 | 1 | drop | 10 (mM) | ||
| 4 | 1 | drop | Tris-HCl | 100 (mM) | pH8.5 |
| 5 | 1 | drop | PEG2000 MME | 8 (%(w/v)) | |
| 6 | 1 | reservoir | PEG2000 MME | 16-25 (%(w/v)) | |
| 7 | 1 | reservoir | 20 (mM) | ||
| 8 | 1 | reservoir | Tris-HCl | 100 (mM) | pH8.5 |
