1PYG
STRUCTURAL BASIS FOR THE ACTIVATION OF GLYCOGEN PHOSPHORYLASE B BY ADENOSINE MONOPHOSPHATE
Experimental procedure
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 169.900, 209.900, 123.400 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 8.000 - 3.000 * |
| R-factor | 0.185 * |
| Rwork | 0.185 |
| RMSD bond length | 0.014 |
| RMSD bond angle | 3.200 |
| Phasing software | X-PLOR |
| Refinement software | X-PLOR |
Data quality characteristics
| Overall | |
| High resolution limit [Å] | 3.000 * |
| Rmerge | 0.053 * |
| Total number of observations | 522198 * |
| Number of reflections | 77728 * |
| Completeness [%] | 99.2 * |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion * | 6.8 * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 5-7 (mg/ml) | |
| 10 | 1 | reservoir | PEG8000 | 8 (%) | |
| 2 | 1 | drop | triethanolamine | 50 (mM) | |
| 3 | 1 | drop | 100 (mM) | ||
| 4 | 1 | drop | ethylenediaminetetraacetic acid | 1 (mM) | |
| 5 | 1 | drop | dithiothreitol | 1 (mM) | |
| 6 | 1 | drop | magnesium acetate | 2.5 (mM) | |
| 7 | 1 | drop | AMP | 0.5 (mM) | |
| 8 | 1 | drop | maltoheptaose | 12.5 (mM) | |
| 9 | 1 | drop | PEG8000 | 3-4 (%) |
