1PYB

Crystal Structure of Aquifex aeolicus Trbp111: a Structure-Specific tRNA Binding Protein

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL7-1
Synchrotron site	SSRL
Beamline	BL7-1
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	1999-08-03
Detector	MARRESEARCH
Wavelength(s)	1.08
Spacegroup name	C 1 2 1
Unit cell lengths	145.400, 72.790, 68.940
Unit cell angles	90.00, 91.93, 90.00

Refinement procedure

Resolution	20.000 - 2.500
R-factor	0.226
Rwork	0.208
R-free	0.22000 *
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1PXF
RMSD bond length	0.005
RMSD bond angle	1.600
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	CNS
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.440
High resolution limit [Å]	2.400	2.400
Rmerge	0.042 *	0.361 *
Number of reflections	26611
<I/σ(I)>	9.7	2.9
Completeness [%]	94.0	88.4
Redundancy	2.1	1.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.2	17 *	PEG2000, ammonium sulfate, imidazole, pH 7.2, VAPOR DIFFUSION, HANGING DROP, temperature 290.15K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	PEG2000	30 (%)
2	1	drop	ammonium sulfate	0.24 (M)
3	1	drop	imidazole	0.1 (M)	pH7.2
4	1	drop	protein	24 (mg/ml)