1PY0
Crystal structure of E51C/E54C Psaz from A.faecalis with CLaNP probe
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU300 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2003-03-16 |
| Detector | MARRESEARCH |
| Spacegroup name | P 65 |
| Unit cell lengths | 42.700, 42.700, 116.900 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 37.010 - 2.000 |
| R-factor | 0.19614 |
| Rwork | 0.193 |
| R-free | 0.23182 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1paz |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.970 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.1.24) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 37.000 | 2.070 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.057 | 0.340 |
| Number of reflections | 39506 | |
| <I/σ(I)> | 25.3 | 5.4 |
| Completeness [%] | 99.2 | 99.1 |
| Redundancy | 4.2 | 4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 291 | 2.4M ammonium sulfate, 50mM sodium phosphate buffer, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
