1PXY
Crystal structure of the actin-crosslinking core of Arabidopsis fimbrin
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X9B |
Synchrotron site | NSLS |
Beamline | X9B |
Temperature [K] | 200 |
Detector technology | CCD |
Collection date | 2001-03-01 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 0.984, 0.9786, 0.9789, 0.9560 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 62.688, 104.981, 104.411 |
Unit cell angles | 90.00, 103.93, 90.00 |
Refinement procedure
Resolution | 20.000 - 2.400 |
R-factor | 0.2293 |
Rwork | 0.229 |
R-free | 0.26990 |
Structure solution method | MAD |
Starting model (for MR) | 1aoa |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | SOLVE |
Refinement software | CNS (1.0) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 2.490 |
High resolution limit [Å] | 2.400 | 2.400 |
Rmerge | 0.315 | |
Number of reflections | 44865 | |
<I/σ(I)> | 20.71 | 3.35 |
Completeness [%] | 87.5 | 86.7 |
Redundancy | 5.97 | 4.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.2 | 298 | 6% PEG 8000, 100 mM Tris, 1mM DTT, pH 8.2, VAPOR DIFFUSION, HANGING DROP, temperature 298K |