1PXU

Crystal structure of chicken NtA from a eukaryotic source at 2.2A resolution

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	EMBL/DESY, HAMBURG BEAMLINE BW7B
Synchrotron site	EMBL/DESY, HAMBURG
Beamline	BW7B
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2002-01-01
Detector	MARRESEARCH
Wavelength(s)	0.8795
Spacegroup name	C 1 2 1
Unit cell lengths	86.685, 50.401, 54.571
Unit cell angles	90.00, 118.93, 90.00

Refinement procedure

Resolution	28.030 - 2.200
R-factor	0.197
Rwork	0.197
R-free	0.23300
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1jb3
RMSD bond length	0.005
RMSD bond angle	1.300
Data reduction software	MOSFLM
Data scaling software	CCP4 ((SCALA))
Phasing software	AMoRE
Refinement software	CNS (1.0)

Data quality characteristics

	Overall
Low resolution limit [Å]	28.000
High resolution limit [Å]	2.200
Number of reflections	10565
Redundancy	2.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.1	292	peg 8000, pH 6.1, VAPOR DIFFUSION, HANGING DROP, temperature 292K