1PXT

THE 2.8 ANGSTROMS STRUCTURE OF PEROXISOMAL 3-KETOACYL-COA THIOLASE OF SACCHAROMYCES CEREVISIAE: A FIVE LAYERED A-B-A-B-A STRUCTURE, CONSTRUCTED FROM TWO CORE DOMAINS OF IDENTICAL TOPOLOGY

Experimental procedure

Spacegroup name	P 21 21 21
Unit cell lengths	71.780, 93.720, 120.450
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	8.000 - 2.800
R-factor	0.198
Rwork	0.198
R-free	0.33400
RMSD bond length	0.012
RMSD bond angle	2.000
Phasing software	X-PLOR
Refinement software	X-PLOR

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000 *
High resolution limit [Å]	2.800 *	2.800 *
Rmerge	0.079 *
Total number of observations	47231 *
Number of reflections	18981 *
Completeness [%]	92.0 *	92.6 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Microdialysis *	7.4 *		THE PROTEIN HAS BEEN CRYSTALLIZED IN THE ABSENCE OF AN ACTIVE SITE LIGAND.

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	1	protein	5 (mg/ml)
2	1	1	potasssium phosphate	200 (mM)
3	1	1	dithiothreitol	1 (mM)
4	1	1		1 (mM)
5	1	1	EDTA	1 (mM)
6	1	2	MOPS	25 (mM)
7	1	2	dithiothreitol	1 (mM)
8	1	2		1 (mM)
9	1	2	EDTA	1 (mM)