1PWO
Crystal Structure of Phospholipase A2 (MIPLA2) from Micropechis Ikaheka
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-BM |
| Synchrotron site | APS |
| Beamline | 19-BM |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2003-01-01 |
| Detector | CUSTOM-MADE |
| Wavelength(s) | 0.97 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 129.186, 100.643, 88.307 |
| Unit cell angles | 90.00, 133.17, 90.00 |
Refinement procedure
| Resolution | 8.000 - 2.600 |
| R-factor | 0.226 |
| Rwork | 0.226 |
| R-free | 0.27100 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | MiPLA4 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.900 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.690 |
| High resolution limit [Å] | 2.600 | 2.600 |
| Number of reflections | 22385 | |
| <I/σ(I)> | 4.52 | |
| Completeness [%] | 97.8 | 99.2 |
| Redundancy | 1.3 | 1.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.6 | 295 | 2.4M Ammonium Sulphate, pH 6.6, VAPOR DIFFUSION, SITTING DROP, temperature 295K |
