1PWH
Rat Liver L-Serine Dehydratase- Complex with PYRIDOXYL-(O-METHYL-SERINE)-5-MONOPHOSPHATE
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2003-04-05 |
| Detector | RIGAKU RAXIS IIC |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 62.241, 109.262, 98.940 |
| Unit cell angles | 90.00, 91.66, 90.00 |
Refinement procedure
| Resolution | 25.000 * - 2.600 |
| R-factor | 0.23 |
| Rwork | 0.230 |
| R-free | 0.26000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | Apo Enzyme 1PWE |
| RMSD bond length | 0.009 |
| RMSD bond angle | 22.800 * |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 35.000 * | 2.690 |
| High resolution limit [Å] | 2.600 | 2.600 |
| Rmerge | 0.078 | 0.124 * |
| Total number of observations | 271545 * | |
| Number of reflections | 40796 * | |
| <I/σ(I)> | 1692.5 | 491.7 |
| Completeness [%] | 95.2 | 75.4 |
| Redundancy | 1.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 22 * | PEG 8000, potassium Acetate, TrisHCL, Dioxane, VAPOR DIFFUSION, HANGING DROP, temperature 22K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | reservoir | HEPES | 50 (mM) | pH8.0 |
| 2 | 1 | reservoir | OMS | 10 (mM) | |
| 3 | 1 | reservoir | potassium acetate | 200 (mM) | |
| 4 | 1 | reservoir | dithiothreitol | 1 (mM) | |
| 5 | 1 | reservoir | PEG8000 | 14 (%(w/v)) | |
| 6 | 1 | reservoir | 1,4-dioxane | 2 (%) | |
| 7 | 1 | drop | protein | 20 (mg/ml) |
