1PWA
Crystal structure of Fibroblast Growth Factor 19
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SRS BEAMLINE PX14.2 |
Synchrotron site | SRS |
Beamline | PX14.2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2003-05-16 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 0.975 |
Spacegroup name | H 3 2 |
Unit cell lengths | 67.573, 67.573, 193.374 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 23.330 - 1.300 |
R-factor | 0.1807 |
Rwork | 0.180 |
R-free | 0.19500 * |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | PDB ENTRIES 1JQZ 2fgf 1ijt 1qqk 1ihk |
RMSD bond length | 0.010 |
RMSD bond angle | 1.440 * |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | AMoRE |
Refinement software | REFMAC (5.1.24) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 23.330 * | 1.320 |
High resolution limit [Å] | 1.300 | 1.300 |
Rmerge | 0.062 * | 0.586 * |
Number of reflections | 42303 * | |
<I/σ(I)> | 36.5 | 4.32 |
Completeness [%] | 99.9 | 100 |
Redundancy | 12.08 | 11.30 * |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 291 | PEG 3350, potassium phosphate, magnesium sulphate, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | Tris-HCl | 10 (mM) | |
2 | 1 | drop | 200 (mM) | ||
3 | 1 | reservoir | PEG3350 | 12-14 (%(w/w)) | |
4 | 1 | reservoir | 0.25 (M) | ||
5 | 1 | reservoir | potassium phosphate | 0.1 (M) | pH7.0 |