1PW4
Crystal Structure of the Glycerol-3-Phosphate Transporter from E.Coli
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X25 |
Synchrotron site | NSLS |
Beamline | X25 |
Detector technology | CCD |
Collection date | 2003-02-19 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.9789, 1.214 |
Spacegroup name | P 32 2 1 |
Unit cell lengths | 97.640, 97.640, 175.151 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 15.000 - 3.300 |
Rwork | 0.296 |
R-free | 0.32530 |
Structure solution method | SIRAS |
RMSD bond length | 0.010 * |
RMSD bond angle | 2.100 * |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | CNS |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 * | 3.360 |
High resolution limit [Å] | 3.300 * | 3.300 |
Rmerge | 0.060 * | 0.386 * |
Number of reflections | 14870 | |
<I/σ(I)> | 11.3 | 2.8 |
Completeness [%] | 99.5 | 93.2 |
Redundancy | 7.7 | 4.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | unknown * | 8.7 | 293 | 25% PEG2K MME, 20% Glycerol, 5% MPD, 0.1M Tris pH8.7, 1mM DTT, 5mM NaAc, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
1 | unknown * | 8.7 | 293 | 25% PEG2K MME, 20% Glycerol, 5% MPD, 0.1M Tris pH8.7, 1mM DTT, 5mM NaAc, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | 1 | protein | 2 (mg/ml) | |
2 | 1 | 1 | dithiothreitol | 5 (mM) |