1PVW

3,4-dihydroxy-2-butanone 4-phosphate synthase from M. jannaschii

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU200
Temperature [K]	287
Detector technology	IMAGE PLATE
Collection date	2001-01-12
Detector	MARRESEARCH
Wavelength(s)	1.548
Spacegroup name	C 2 2 21
Unit cell lengths	66.315, 154.205, 133.152
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	20.000 - 2.450
R-factor	0.235
Rwork	0.229
R-free	0.28900
Structure solution method	MIR
RMSD bond length	0.008
RMSD bond angle	1.240
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	SHELXS
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	2.540
High resolution limit [Å]	2.450	2.450
Rmerge	0.065	0.295
Number of reflections	24943
<I/σ(I)>	21.5	3.5
Completeness [%]	97.8	97.3
Redundancy	4.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	unknown *	7.5	293	Fischer, M., (2002) J. Biol. Chem., 277, 41410. *