1PVU
THE CRYSTAL STRUCTURE OF PVUII ENDONUCLEASE REVEALS EXTENSIVE STRUCTURAL HOMOLOGIES TO ECORV
Experimental procedure
Spacegroup name | P 21 21 2 |
Unit cell lengths | 84.650, 106.460, 47.000 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 6.000 - 2.400 |
R-factor | 0.184 |
Rwork | 0.184 |
RMSD bond length | 0.023 |
RMSD bond angle | 3.620 |
Phasing software | X-PLOR |
Refinement software | X-PLOR |
Data quality characteristics
Overall | |
High resolution limit [Å] | 2.300 * |
Rmerge | 0.076 * |
Number of reflections | 16315 * |
Completeness [%] | 96.3 * |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion, hanging drop * | 5 * | 18 * | Athanasiadis, A., (1991) J.Mol.Biol., 222, 451. * |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein solution | 2.5 (mg/ml) | |
2 | 1 | drop | ammonium acetate | 100 (mM) | |
3 | 1 | drop | ammonium sulfate | 10-20 (%) | |
4 | 1 | reservoir | ammonium sulfate | 44 (%) |