1PVM
Crystal Structure of a Conserved CBS Domain Protein TA0289 of Unknown Function from Thermoplasma acidophilum
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-ID |
Synchrotron site | APS |
Beamline | 19-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2003-06-17 |
Detector | SBC-2 |
Wavelength(s) | 1.013 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 58.735, 62.838, 97.278 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 50.000 * - 1.500 |
R-factor | 0.194 |
Rwork | 0.194 |
R-free | 0.22800 |
Structure solution method | SAD |
RMSD bond length | 0.005 |
RMSD bond angle | 23.300 * |
Data reduction software | d*TREK |
Data scaling software | HKL-2000 |
Phasing software | CNS |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.550 |
High resolution limit [Å] | 1.500 | 1.500 |
Rmerge | 0.056 | 0.217 |
Number of reflections | 105550 | |
<I/σ(I)> | 44.06 | 5.04 |
Completeness [%] | 97.0 | 82.5 |
Redundancy | 10.5 | 5.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion * | 7.8 * | 298 | 0.1M tris, 0.2M ammonium formate, 8% Glycerol, 24%PEG3350, 1.6M Mercury sulphate in protein,, pH 6.4, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | reservoir | Tris-HCl | 0.1 (M) | pH7.8 |
2 | 1 | reservoir | ammonium formate | 0.2 (M) | |
3 | 1 | reservoir | PEG3350 | 20 (%) | |
4 | 1 | reservoir | glycerol | 8 (%) |