1PVD
CRYSTAL STRUCTURE OF THE THIAMIN DIPHOSPHATE DEPENDENT ENZYME PYRUVATE DECARBOXYLASE FROM THE YEAST SACCHAROMYCES CEREVISIAE AT 2.3 ANGSTROMS RESOLUTION
Experimental procedure
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 142.060, 74.750, 120.320 |
| Unit cell angles | 90.00, 116.58, 90.00 |
Refinement procedure
| Resolution | 10.000 - 2.300 |
| R-factor | 0.165 |
| Rwork | 0.165 |
| RMSD bond length | 0.018 |
| RMSD bond angle | 3.100 |
| Phasing software | X-PLOR |
| Refinement software | GPRLSA |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 40.000 |
| High resolution limit [Å] | 2.300 |
| Rmerge | 0.080 * |
| Total number of observations | 199754 * |
| Number of reflections | 46787 |
| Completeness [%] | 93.0 * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 4 (mg/ml) | |
| 2 | 1 | drop | PEG | 10 (%(w/v)) | |
| 3 | 1 | drop | citric acid/sodium citrate buffer | 0.01 (M) | |
| 4 | 1 | drop | thiamin diphosphate | 0.002 (M) | |
| 5 | 1 | drop | 0.002 (M) | ||
| 6 | 1 | drop | dithiothreitol | 0.001 (M) | |
| 7 | 1 | reservoir | PEG8000 |
