1PVD

CRYSTAL STRUCTURE OF THE THIAMIN DIPHOSPHATE DEPENDENT ENZYME PYRUVATE DECARBOXYLASE FROM THE YEAST SACCHAROMYCES CEREVISIAE AT 2.3 ANGSTROMS RESOLUTION

Experimental procedure

Spacegroup name	C 1 2 1
Unit cell lengths	142.060, 74.750, 120.320
Unit cell angles	90.00, 116.58, 90.00

Refinement procedure

Resolution	10.000 - 2.300
R-factor	0.165
Rwork	0.165
RMSD bond length	0.018
RMSD bond angle	3.100
Phasing software	X-PLOR
Refinement software	GPRLSA

Data quality characteristics

	Overall
Low resolution limit [Å]	40.000
High resolution limit [Å]	2.300
Rmerge	0.080 *
Total number of observations	199754 *
Number of reflections	46787
Completeness [%]	93.0 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	4 (mg/ml)
2	1	drop	PEG	10 (%(w/v))
3	1	drop	citric acid/sodium citrate buffer	0.01 (M)
4	1	drop	thiamin diphosphate	0.002 (M)
5	1	drop		0.002 (M)
6	1	drop	dithiothreitol	0.001 (M)
7	1	reservoir	PEG8000