1PVB
X-RAY STRUCTURE OF A NEW CRYSTAL FORM OF PIKE 4.10 PARVALBUMIN
Experimental procedure
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 51.840, 49.950, 34.960 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 8.000 - 1.750 |
| R-factor | 0.168 |
| Rwork | 0.168 |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.360 |
| Phasing software | X-PLOR |
| Refinement software | X-PLOR |
Data quality characteristics
| Overall | |
| High resolution limit [Å] | 1.750 * |
| Rmerge | 0.051 * |
| Total number of observations | 18280 * |
| Number of reflections | 9484 |
| Completeness [%] | 98.0 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 27 (mg/ml) | |
| 2 | 1 | drop | ammonium sulfate | 2 (M) | |
| 3 | 1 | drop | EDTA | 1 (mM) | |
| 4 | 1 | drop | sodium azide | 3 (mM) | |
| 5 | 1 | drop | Tris-HCl | ||
| 6 | 1 | reservoir | ammonium sulfate | 2.4 (M) | |
| 7 | 1 | reservoir | EDTA | 1 (mM) | |
| 8 | 1 | reservoir | sodium azide | 3 (mM) | |
| 9 | 1 | reservoir | Tris-HCl |
