1PUM

Mistletoe lectin I in complex with galactose

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	EMBL/DESY, HAMBURG BEAMLINE X11
Synchrotron site	EMBL/DESY, HAMBURG
Beamline	X11
Temperature [K]	100
Detector technology	CCD
Collection date	2000-04-18
Detector	MARRESEARCH
Wavelength(s)	0.9073
Spacegroup name	P 65 2 2
Unit cell lengths	106.826, 106.826, 310.951
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	10.000 - 2.300
R-factor	0.28
Rwork	0.209
R-free	0.24600
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond angle	1.569
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	25.000	25.000
High resolution limit [Å]	2.300	2.300
Rmerge	0.048	0.423
Number of reflections	46562
<I/σ(I)>	21.2	2.58
Completeness [%]	96.5	96.3
Redundancy	11	4.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	2.5	293	ammonium sulphate, glycin-HCL, galactose, dioxan, pH 2.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K