1PUJ

Structure of B. subtilis YlqF GTPase

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X4A
Synchrotron site	NSLS
Beamline	X4A
Temperature [K]	100
Detector technology	CCD
Collection date	2003-03-01
Detector	ADSC QUANTUM 4
Wavelength(s)	0.9798
Spacegroup name	P 21 21 21
Unit cell lengths	36.749, 68.568, 105.574
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.410 - 2.000
R-factor	0.2161
Rwork	0.216
R-free	0.25000
Structure solution method	SAD
RMSD bond length	0.005
RMSD bond angle	1.100
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	SOLVE
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	2.070
High resolution limit [Å]	2.000	2.000
Rmerge	0.074
Number of reflections	18735
Completeness [%]	99.9	99.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	6.5	291	11-13% PEG 8000, 0.1M Na Cacodylate pH 6.5 3% MPD, VAPOR DIFFUSION, temperature 291K