1PUD
TRNA-GUANINE TRANSGLYCOSYLASE
Experimental procedure
| Source type | SYNCHROTRON |
| Source details | LURE |
| Synchrotron site | LURE |
| Detector technology | IMAGE PLATE |
| Detector | MARRESEARCH |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 92.100, 65.100, 71.900 |
| Unit cell angles | 90.00, 97.50, 90.00 |
Refinement procedure
| Resolution | 8.000 - 1.850 |
| R-factor | 0.19 |
| Rwork | 0.190 |
| R-free | 0.21400 |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.550 |
| Data reduction software | XDS |
| Phasing software | X-PLOR |
| Refinement software | X-PLOR |
Data quality characteristics
| Overall | |
| High resolution limit [Å] | 1.850 * |
| Rmerge | 0.029 |
| Total number of observations | 82096 * |
| Number of reflections | 33727 |
| Completeness [%] | 93.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 7.5 * | 25 * | Romier, C., (1996) Proteins: Struct.,Funct., Genet., 24, 516. * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | reservoir | Tris-HCl | 100 (mM) | |
| 2 | 1 | reservoir | PEG8000 | 13 (%) | |
| 3 | 1 | reservoir | dithiothreitol | 1 (mM) | |
| 4 | 1 | reservoir | agarose | 0.2 (%) | |
| 5 | 1 | drop | HEPES | 10 (mM) | pH7.5 |
| 6 | 1 | drop | 2 (M) | ||
| 7 | 1 | drop | dithiothreitol | 1 (mM) | |
| 8 | 1 | drop | protein | 12 (mg/ml) |
