1PUC

P13SUC1 IN A STRAND-EXCHANGED DIMER

Experimental procedure

Detector technology	AREA DETECTOR
Collection date	1994-09-28
Detector	XUONG-HAMLIN MULTIWIRE
Wavelength(s)	1.5418
Spacegroup name	C 2 2 21
Unit cell lengths	43.270, 55.280, 111.800
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	6.000 - 1.950
R-factor	0.187
R-free	0.24100
RMSD bond length	0.006
RMSD bond angle	24.472 *
Data reduction software	SDMS
Phasing software	X-PLOR (3.1)
Refinement software	TNT

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000 *
High resolution limit [Å]	1.950	1.950 *
Rmerge	0.049
Total number of observations	53600 *
Number of reflections	9859
Completeness [%]	97.5	81.4 *
Redundancy	5.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, sitting drop *	7.8 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	recombinant p13suc1	12 (mg/ml)
2	1	drop		38 (mM)
3	1	drop	Tris-HCl	38 (mM)
4	1	drop	PEG MME 2000	9 (%(w/v))
5	1	drop	CHAPS	0.4 (%(w/v))
6	1	reservoir	PEG MME 2000	25 (%(w/v))
7	1	reservoir		0.1 (M)
8	1	reservoir	Tris-HCl	0.1 (M)