1PU9
Crystal Structure of Tetrahymena GCN5 with Bound Coenzyme A and a 19-residue Histone H3 Peptide
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | CHESS BEAMLINE A1 |
Synchrotron site | CHESS |
Beamline | A1 |
Detector technology | CCD |
Collection date | 2000-06-08 |
Detector | ADSC QUANTUM 210 |
Spacegroup name | P 32 2 1 |
Unit cell lengths | 64.730, 64.730, 96.416 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 50.000 * - 2.300 |
R-factor | 0.236 |
Rwork | 0.236 |
R-free | 0.27700 * |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1qsn |
RMSD bond length | 0.021 * |
RMSD bond angle | 2.640 * |
Data reduction software | MOSFLM |
Data scaling software | CCP4 ((SCALA)) |
Phasing software | AMoRE |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 * | 2.390 |
High resolution limit [Å] | 2.210 * | 2.210 * |
Rmerge | 0.092 * | 0.180 * |
Total number of observations | 67990 * | |
Number of reflections | 22051 * | |
Completeness [%] | 99.5 * | 99.5 * |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion, hanging drop * | 7.5 | 20 * | Ammonium sulfate, MnCl2, Tris-HCl, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K, pH 7.50 |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | tGCN5 | 0.4 (mM) | |
2 | 1 | drop | CoA | 1.0 (mM) | |
3 | 1 | drop | H3-derived peptide | 1.5 (mM) | |
4 | 1 | reservoir | ammonium sulfate | 1.6 (M) | |
5 | 1 | reservoir | 10-50 (mM) | ||
6 | 1 | reservoir | Tris | 50 (mM) | pH7.5 |