1PU5

GM2-activator Protein crystal structure

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 17-BM
Synchrotron site	APS
Beamline	17-BM
Temperature [K]	123
Wavelength(s)	0.979
Spacegroup name	P 21 21 21
Unit cell lengths	63.320, 86.130, 120.210
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	7.990 - 1.900
R-factor	0.214
Rwork	0.214
R-free	0.25300
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1g13
RMSD bond length	0.011
RMSD bond angle	1.600
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Refinement software	CNS (1.0)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.970
High resolution limit [Å]	1.900	1.900
Rmerge	0.049	0.515
Number of reflections	50312
<I/σ(I)>	5
Completeness [%]	97.2
Redundancy	3.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.7	278	Peg 4000, iso-propanol, Hepes buffer, pH 7.7, VAPOR DIFFUSION, HANGING DROP, temperature 278.0K