1PTO
THE STRUCTURE OF A PERTUSSIS TOXIN-SUGAR COMPLEX AS A MODEL FOR RECEPTOR BINDING
Experimental procedure
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 163.800, 98.200, 194.500 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 10.000 - 3.500 |
| R-factor | 0.183 |
| Rwork | 0.183 |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.900 |
| Phasing software | X-PLOR |
| Refinement software | X-PLOR |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 10.000 * |
| High resolution limit [Å] | 3.500 * |
| Rmerge | 0.097 * |
| Completeness [%] | 87.0 * |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 8 * | Stein, P.E., (1994) Structure, 2, 45. * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | reservoir | sodium potassium phosphate | 25 (mM) | |
| 2 | 1 | reservoir | 0.25 (M) | ||
| 3 | 1 | reservoir | 0.02 (%) | ||
| 4 | 1 | reservoir | 0.3-0.5 (M) | precipitant |
