1PTF

THE 1.6 ANGSTROMS STRUCTURE OF HISTIDINE-CONTAINING PHOSPHOTRANSFER PROTEIN HPR FROM STREPTOCOCCUS FAECALIS

Experimental procedure

Spacegroup name	P 21 21 2
Unit cell lengths	53.560, 45.460, 29.880
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	8.000 - 1.600
R-factor	0.156
Rwork	0.156
RMSD bond length	0.014
RMSD bond angle	1.900
Phasing software	X-PLOR
Refinement software	PROLSQ

Data quality characteristics

	Overall
High resolution limit [Å]	1.600 *
Rmerge	0.061 *
Total number of observations	88281 *
Number of reflections	9587 *
Completeness [%]	95.0 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	5 *	11 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	2.7 (mg/ml)
2	1	drop		45 (%sat)
3	1	reservoir		50 (%sat)
4	1	reservoir	citrate-phosphate buffer