1PT6
I domain from human integrin alpha1-beta1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE X11 |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | X11 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2002-10-04 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.8110 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 37.471, 97.601, 53.094 |
| Unit cell angles | 90.00, 103.63, 90.00 |
Refinement procedure
| Resolution | 24.250 - 1.870 |
| R-factor | 0.19369 |
| Rwork | 0.191 |
| R-free | 0.24020 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1gcy |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.318 |
| Data scaling software | XDS |
| Phasing software | CNS |
| Refinement software | REFMAC (5.1.29) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.000 | 2.000 |
| High resolution limit [Å] | 1.870 | 1.870 |
| Rmerge | 0.056 | 0.294 |
| Number of reflections | 29394 | |
| <I/σ(I)> | 15.6 | 4.5 |
| Completeness [%] | 95.8 | 88.7 |
| Redundancy | 3.7 | 3.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 295 | PEG 6000, tris, sodium acetate, glycerol, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 295K |
