1PT5
Crystal structure of gene yfdW of E. coli
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID14-4 |
Synchrotron site | ESRF |
Beamline | ID14-4 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2002-09-08 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 0.9330 |
Spacegroup name | P 62 |
Unit cell lengths | 146.882, 146.882, 129.515 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 20.000 - 2.000 |
R-factor | 0.1592 |
Rwork | 0.158 |
R-free | 0.16800 * |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | apo-enzyme |
RMSD bond length | 0.034 * |
RMSD bond angle | 2.000 * |
Data reduction software | DENZO |
Data scaling software | CCP4 ((SCALA)) |
Phasing software | AMoRE |
Refinement software | REFMAC (5.1.19) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 2.110 |
High resolution limit [Å] | 2.000 | 2.000 |
Rmerge | 0.078 * | 0.265 * |
Number of reflections | 105732 | |
<I/σ(I)> | 7 | |
Completeness [%] | 99.0 | 97.5 |
Redundancy | 6.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 7.5 * | 298 | PEG600, cacodylate, acetylCoA, pH 6.5, VAPOR DIFFUSION, temperature 298.0K |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 11 (mg/ml) | |
2 | 1 | drop | sodium HEPES | 5 (mM) | |
3 | 1 | drop | 150 (mM) | ||
4 | 1 | reservoir | ammonium phosphate | 0.9 (M) | |
5 | 1 | reservoir | sodium HEPES | 0.1 (M) | pH7.5 |