1PSW
Structure of E. coli ADP-heptose lps heptosyltransferase II
Experimental procedure
Experimental method | SAD |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 31-ID |
Synchrotron site | APS |
Beamline | 31-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2003-06-07 |
Detector | MARRESEARCH |
Wavelength(s) | 0.9798 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 63.407, 150.125, 49.674 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 19.960 - 2.000 |
R-factor | 0.2011 |
Rwork | 0.201 |
R-free | 0.22500 |
Structure solution method | SAD |
RMSD bond length | 0.005 |
RMSD bond angle | 1.200 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | SOLVE |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 1.970 |
High resolution limit [Å] | 1.900 | 1.900 |
Rmerge | 0.093 | |
Number of reflections | 37529 | |
Completeness [%] | 99.8 | 100 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 5.6 | 291 | 1M Ammonium Phospate, 0.1M, Na Citrate pH 5.6 , VAPOR DIFFUSION, temperature 291K |