1PSO
The crystal structure of human pepsin and its complex with pepstatin
Experimental procedure
Source type | ROTATING ANODE |
Detector technology | AREA DETECTOR |
Collection date | 1994-06-17 |
Detector | SAN DIEGO MULTIWIRE DETECTION SYSTEM |
Wavelength(s) | 1.5418 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 72.068, 150.972, 40.854 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 30.000 - 2.000 |
Rwork | 0.185 |
RMSD bond length | 0.011 |
RMSD bond angle | 2.750 * |
Data reduction software | SAN (DIEGO SOFTWARE) |
Refinement software | GROMOS |
Data quality characteristics
Overall | |
High resolution limit [Å] | 1.970 * |
Rmerge | 0.054 * |
Total number of observations | 166144 * |
Number of reflections | 31184 |
Completeness [%] | 97.0 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion, hanging drop * |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | pepsin | 20 (mg/ml) | |
2 | 1 | drop | pepstasin | 5 (mM) | |
3 | 1 | drop | methanol |