1PSD
THE ALLOSTERIC LIGAND SITE IN THE VMAX-TYPE COOPERATIVE ENZYME PHOSPHOGLYCERATE DEHYDROGENASE
Experimental procedure
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 136.100, 146.300, 53.300 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 9.000 - 2.750 |
| R-factor | 0.177 |
| Rwork | 0.177 |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.580 |
| Phasing software | X-PLOR |
| Refinement software | X-PLOR |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 50.000 |
| High resolution limit [Å] | 2.720 |
| Rmerge | 0.119 * |
| Total number of observations | 287548 * |
| Number of reflections | 26417 |
| Completeness [%] | 84.0 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | reservoir | sodium citrate | 400 (mM) | |
| 10 | 1 | drop | PEG3350 | 14 (%) | |
| 2 | 1 | reservoir | PEG3350 | 14 (%) | |
| 3 | 1 | drop | PGDH | 10 (mg/ml) | |
| 4 | 1 | drop | serine | 4 (mM) | |
| 5 | 1 | drop | NAD | 4 (mM) | |
| 6 | 1 | drop | DTT | 2 (mM) | |
| 7 | 1 | drop | EDTA | 1 (mM) | |
| 8 | 1 | drop | sodium azide | 0.05 (%) | |
| 9 | 1 | drop | sodium citrate | 400 (mM) |
