1PSD
THE ALLOSTERIC LIGAND SITE IN THE VMAX-TYPE COOPERATIVE ENZYME PHOSPHOGLYCERATE DEHYDROGENASE
Experimental procedure
Spacegroup name | P 21 21 2 |
Unit cell lengths | 136.100, 146.300, 53.300 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 9.000 - 2.750 |
R-factor | 0.177 |
Rwork | 0.177 |
RMSD bond length | 0.008 |
RMSD bond angle | 1.580 |
Phasing software | X-PLOR |
Refinement software | X-PLOR |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 50.000 |
High resolution limit [Å] | 2.720 |
Rmerge | 0.119 * |
Total number of observations | 287548 * |
Number of reflections | 26417 |
Completeness [%] | 84.0 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion, hanging drop * |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | reservoir | sodium citrate | 400 (mM) | |
10 | 1 | drop | PEG3350 | 14 (%) | |
2 | 1 | reservoir | PEG3350 | 14 (%) | |
3 | 1 | drop | PGDH | 10 (mg/ml) | |
4 | 1 | drop | serine | 4 (mM) | |
5 | 1 | drop | NAD | 4 (mM) | |
6 | 1 | drop | DTT | 2 (mM) | |
7 | 1 | drop | EDTA | 1 (mM) | |
8 | 1 | drop | sodium azide | 0.05 (%) | |
9 | 1 | drop | sodium citrate | 400 (mM) |