1PRZ
Crystal structure of pseudouridine synthase RluD catalytic module
Experimental procedure
| Experimental method | MAD |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2003-02-24 |
| Detector | RIGAKU RAXIS HTC |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 52.261, 76.362, 85.717 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 45.000 * - 1.800 |
| Rwork | 0.218 |
| R-free | 0.24300 |
| Structure solution method | MAD |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.800 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SOLVE |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.860 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.065 * | |
| Total number of observations | 107285 * | |
| Number of reflections | 32444 | |
| <I/σ(I)> | 12.4 | |
| Completeness [%] | 99.4 | 98.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Batch method * | 8 * | 4 * | PEG 8000, KCL, Glycerol, pH 7., microbatch under oil, temperature 293K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | 2 | PEG8000 | 14 (%(w/v)) | |
| 2 | 1 | 2 | 550 (mM) | ||
| 3 | 1 | 2 | glycerol | 12 (%) | |
| 4 | 1 | 1 | protein | 14 (mg/ml) | |
| 5 | 1 | 1 | Tris-HCl | 20 (mM) | pH8. |
| 6 | 1 | 1 | 200 (mM) | ||
| 7 | 1 | 1 | dithiothreitol | 10 (mM) | |
| 8 | 1 | 1 | glycerol | 5 (%) |
