1PRY

Structure Determination of Fibrillarin Homologue From Hyperthermophilic Archaeon Pyrococcus furiosus (Pfu-65527)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 17-ID
Synchrotron site	APS
Beamline	17-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2001-10-16
Detector	MARRESEARCH
Wavelength(s)	1.74
Spacegroup name	P 21 21 21
Unit cell lengths	43.967, 62.082, 103.363
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	40.060 * - 1.970
R-factor	0.228 *
Rwork	0.204
R-free	0.27200 *
Structure solution method	Single Anomalous Scattering
RMSD bond length	0.006 *
RMSD bond angle	1.300 *
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.460	2.050
High resolution limit [Å]	1.970	1.970
Rmerge	0.052 *
Number of reflections	18879
<I/σ(I)>	19.4
Completeness [%]	82.3 *	33.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Batch method *	8.5	18 *	PEG 6000, ammonium phosphate, pH 8.5, Microbatch, temperature 291K
1	Batch method *	8.5	18 *	PEG 6000, ammonium phosphate, pH 8.5, Microbatch, temperature 291K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	1	PEG8000	10 (%)
2	1	1	calcium acetate	200 (mM)
3	1	1	imidazole	100 (mM)	pH8.0
4	1	1	PEG6000	12 (%)
5	1	1	di-ammonium hydrogen phosphate	100 (mM)
6	1	1	Tris-HCl	100 (mM)	pH8.5