1PRY
Structure Determination of Fibrillarin Homologue From Hyperthermophilic Archaeon Pyrococcus furiosus (Pfu-65527)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 17-ID |
| Synchrotron site | APS |
| Beamline | 17-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2001-10-16 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.74 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 43.967, 62.082, 103.363 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 40.060 * - 1.970 |
| R-factor | 0.228 * |
| Rwork | 0.204 |
| R-free | 0.27200 * |
| Structure solution method | Single Anomalous Scattering |
| RMSD bond length | 0.006 * |
| RMSD bond angle | 1.300 * |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.460 | 2.050 |
| High resolution limit [Å] | 1.970 | 1.970 |
| Rmerge | 0.052 * | |
| Number of reflections | 18879 | |
| <I/σ(I)> | 19.4 | |
| Completeness [%] | 82.3 * | 33.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Batch method * | 8.5 | 18 * | PEG 6000, ammonium phosphate, pH 8.5, Microbatch, temperature 291K |
| 1 | Batch method * | 8.5 | 18 * | PEG 6000, ammonium phosphate, pH 8.5, Microbatch, temperature 291K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | 1 | PEG8000 | 10 (%) | |
| 2 | 1 | 1 | calcium acetate | 200 (mM) | |
| 3 | 1 | 1 | imidazole | 100 (mM) | pH8.0 |
| 4 | 1 | 1 | PEG6000 | 12 (%) | |
| 5 | 1 | 1 | di-ammonium hydrogen phosphate | 100 (mM) | |
| 6 | 1 | 1 | Tris-HCl | 100 (mM) | pH8.5 |
