1PRQ

ACANTHAMOEBA CASTELLANII PROFILIN IA

Experimental procedure

Source type	ROTATING ANODE
Temperature [K]	290
Detector technology	AREA DETECTOR
Collection date	1991
Detector	SIEMENS
Spacegroup name	C 1 2 1
Unit cell lengths	91.090, 37.400, 34.700
Unit cell angles	90.00, 109.60, 90.00

Refinement procedure

Resolution	8.000 - 2.500
Rwork	0.141
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	REFINED ACANTHAMOEBA PROFILIN IB STRUCTURE (1ACF)
RMSD bond length	0.009
RMSD bond angle	0.028
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	X-PLOR
Refinement software	PROFFT

Data quality characteristics

	Overall
Low resolution limit [Å]	43.100
High resolution limit [Å]	2.500
Rmerge	0.049
Number of reflections	3255
<I/σ(I)>	19.9
Completeness [%]	82.8
Redundancy	2.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		8		1.5 M SODIUM POTASSIUM TARTRATE, pH 8.0