1PQZ
MURINE CYTOMEGALOVIRUS IMMUNOMODULATORY PROTEIN M144
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 117 |
| Detector technology | IMAGE PLATE |
| Collection date | 2002-11-18 |
| Detector | RIGAKU RAXIS IV |
| Wavelength(s) | 1.5418 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 128.800, 50.600, 71.200 |
| Unit cell angles | 90.00, 104.10, 90.00 |
Refinement procedure
| Resolution | 19.720 - 2.100 |
| R-factor | 0.245 |
| Rwork | 0.245 |
| R-free | 0.28700 |
| Structure solution method | MIR |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.400 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MLPHARE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.140 |
| High resolution limit [Å] | 2.050 | 2.050 |
| Rmerge | 0.052 | 0.404 |
| <I/σ(I)> | 18.61 | 2.16 |
| Completeness [%] | 95.9 | 95.2 |
| Redundancy | 2.57 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.8 | 293 | 16% PEG 8k 100mM cacodylate pH 5.8, .02% Ethyl Acetate, 150mM NaCl, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
