1PQO

T4 Lysozyme Core Repacking Mutant L118I/TA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2001-11-10
Detector	RIGAKU RAXIS IV
Spacegroup name	P 32 2 1
Unit cell lengths	60.181, 60.181, 95.862
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	18.700 - 1.650
R-factor	0.207
Rwork	0.204
R-free	0.26100
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1l63
RMSD bond length	0.013
RMSD bond angle	2.300 *
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	TNT
Refinement software	TNT

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	18.700	1.720
High resolution limit [Å]	1.650	1.650
Rmerge	0.049	0.300
Number of reflections	23077
<I/σ(I)>	7.9	2.4
Completeness [%]	94.0	91 *
Redundancy	2	3.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion *	6.7	277	Eriksson, A.E., (1993) J.Mol.Biol., 229, 747. *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	20 (mg/ml)
2	1	reservoir		0.25 (M)
3	1	reservoir	phosphate	2.0 (M)
4	1	reservoir	beta-mercaptoethanol